Multi-point scanning two-photon excitation microscopy by utilizing a high-peak-power 1042-nm laser.
نویسندگان
چکیده
The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
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ورودعنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 31 4 شماره
صفحات -
تاریخ انتشار 2015